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gene tissue expression data gtex  (Human Protein Atlas)
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Human Protein Atlas gene tissue expression data gtex
a Reaggregation of iPSc-derived TEPs with primary human ETPs to form hTOs (human Thymic Organoids) cultured in a 3D fibrin hydrogel at an air-liquid interface in medium supplemented with RANKL, IL7, SCF, and FLT3L (Created in BioRender. Guillonneau, C. <t>(2025)</t> <t>https://BioRender.com/apk8n61</t> ). b Bright-field images of hTOs (human Thymic Organoids) at 24 h and D4 showing a compact spheroid core with a peripheral cellular crown (identical magnification) ( n = 12 from independent differentiations and ETP donors). c scRNA-seq of EPCAM + CD45 − cells from D7 organoids with UMAP embedding and cell-cycle scoring (10x Genomics; Seurat workflow). d Module score for a TEP gene set: PDPN , PAX9 , IL7 , EBF1 , FN1 . e Integration of the EPCAM + CD45 − scRNA-seq dataset (Clusters 1, 2, and 3) with the Bautista human TEC atlas; with reference cells in gray. f Cell type deconvolution of TEPs and CD205 + TEPs against the EPCAM + CD45 − scRNA-seq dataset. g Distribution of the Tau tissue-specific variability index within clusters 3 and 1. Tau was computed from <t>GTEx</t> tissue expression data; TRA enrichment was assessed on the top-200 cluster-specific genes. Source data are provided as a file. h Assessment of the TRA repertoire in Cluster 3, showing enrichment for brain antigens. Source data are provided as a file.
Gene Tissue Expression Data Gtex, supplied by Human Protein Atlas, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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a Reaggregation of iPSc-derived TEPs with primary human ETPs to form hTOs (human Thymic Organoids) cultured in a 3D fibrin hydrogel at an air-liquid interface in medium supplemented with RANKL, IL7, SCF, and FLT3L (Created in BioRender. Guillonneau, C. (2025) https://BioRender.com/apk8n61 ). b Bright-field images of hTOs (human Thymic Organoids) at 24 h and D4 showing a compact spheroid core with a peripheral cellular crown (identical magnification) ( n = 12 from independent differentiations and ETP donors). c scRNA-seq of EPCAM + CD45 − cells from D7 organoids with UMAP embedding and cell-cycle scoring (10x Genomics; Seurat workflow). d Module score for a TEP gene set: PDPN , PAX9 , IL7 , EBF1 , FN1 . e Integration of the EPCAM + CD45 − scRNA-seq dataset (Clusters 1, 2, and 3) with the Bautista human TEC atlas; with reference cells in gray. f Cell type deconvolution of TEPs and CD205 + TEPs against the EPCAM + CD45 − scRNA-seq dataset. g Distribution of the Tau tissue-specific variability index within clusters 3 and 1. Tau was computed from GTEx tissue expression data; TRA enrichment was assessed on the top-200 cluster-specific genes. Source data are provided as a file. h Assessment of the TRA repertoire in Cluster 3, showing enrichment for brain antigens. Source data are provided as a file.

Journal: Nature Communications

Article Title: Combinatory differentiation of human induced pluripotent stem cells generates functional thymic epithelium driving dendritic cell and CD4/CD8 T cell development

doi: 10.1038/s41467-026-68675-y

Figure Lengend Snippet: a Reaggregation of iPSc-derived TEPs with primary human ETPs to form hTOs (human Thymic Organoids) cultured in a 3D fibrin hydrogel at an air-liquid interface in medium supplemented with RANKL, IL7, SCF, and FLT3L (Created in BioRender. Guillonneau, C. (2025) https://BioRender.com/apk8n61 ). b Bright-field images of hTOs (human Thymic Organoids) at 24 h and D4 showing a compact spheroid core with a peripheral cellular crown (identical magnification) ( n = 12 from independent differentiations and ETP donors). c scRNA-seq of EPCAM + CD45 − cells from D7 organoids with UMAP embedding and cell-cycle scoring (10x Genomics; Seurat workflow). d Module score for a TEP gene set: PDPN , PAX9 , IL7 , EBF1 , FN1 . e Integration of the EPCAM + CD45 − scRNA-seq dataset (Clusters 1, 2, and 3) with the Bautista human TEC atlas; with reference cells in gray. f Cell type deconvolution of TEPs and CD205 + TEPs against the EPCAM + CD45 − scRNA-seq dataset. g Distribution of the Tau tissue-specific variability index within clusters 3 and 1. Tau was computed from GTEx tissue expression data; TRA enrichment was assessed on the top-200 cluster-specific genes. Source data are provided as a file. h Assessment of the TRA repertoire in Cluster 3, showing enrichment for brain antigens. Source data are provided as a file.

Article Snippet: The Tau was calculated for a list of genes using the gene tissue expression data (GTEx) from the Human Protein Atlas database ( https://www.proteinatlas.org/about/download ).

Techniques: Derivative Assay, Cell Culture, Expressing